Who invented northern blot

Clinical Chemistry Neal Burnette: The man behind the Western blot. January pg Burnette 10 Eisenstein, M: A look back: westward expansion. Nature Methods. Click here to see the Eisenstein 'Classic Protocol' story Please contact us if you have any questions or comments. Best of luck and enjoy getting PerfectWestern blots! Site Information.

Please wait Sign in or Create an account. Home Who really invented the Western Blot? Do you know who invented the Western blot? Learn the actual story behind the western blotting technique Was it Neal Burnette at Fred Hutchinson?

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Learning Objectives Evaluate the applications of Northern Blots. Because there are so many different RNA molecules on the gel, it usually appears as a smear rather than discrete bands.

The RNA is transfered to a sheet of special blotting paper called nitrocellulose, though other types of paper, or membranes, can be used. The RNA molecules retain the same pattern of separation they had on the gel. The blot is incubated with a probe which is single-stranded DNA. So typically you would isolate a population of RNA from some cell sample or tissue sample, and then you would electrophoretically allow the RNA to migrate down a gel. So you're going to have the smaller fragments at the bottom and the larger fragments at the top.

Once you've finished what we call running the gel, you apply a membrane to the gel and transfer, either by a salt gradient or by electrophoretic transfer, the molecules, the RNA molecules, in the gel to a membrane, typically nitrocellulose.

Having that on nitrocellulose is going to look mostly like a white piece of paper. Having that, you're going to be able to then interrogate the differences in the RNA samples. We can ask if some RNA is gone or not by marking that RNA and then finding its match on the northern blot membrane, or you can ask, does it change in size? You might expect a change in size if you had an alteration in RNA splicing.